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Merck & Co pgl-ap1-luc
Pgl Ap1 Luc, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl-ap1-luc/pgl+ap1+luc/10__1074_slash_jbc__274__28__19992-97-27-33
Average 90 stars, based on 1 article reviews
pgl-ap1-luc - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Activity Assay:

Article Title: Gi Proteins Use a Novel βγ- and Ras-independent Pathway to Activate Extracellular Signal-regulated Kinase and Mobilize AP-1 Transcription Factors in Jurkat T Lymphocytes
Article Snippet: AP-1 Reporter Activity Assay—Cells (107) in Medium B were transfected with 10 mg of irrelevant plasmid DNA (pcDNAIII; Invitrogen) and 20 mg of the AP-1 reporter plasmid, pGL-AP1-Luc (generously provided by E. O’Neill (Merck)), which contains three tandem repeats of a sequence (59-GTGACTCAGCGCG-39) with a high affinity AP-1 binding site (underlined) upstream of a minimal g-fibrinogen promoter (46) and a luciferase gene.

Transfection:

Article Title: Gi Proteins Use a Novel βγ- and Ras-independent Pathway to Activate Extracellular Signal-regulated Kinase and Mobilize AP-1 Transcription Factors in Jurkat T Lymphocytes
Article Snippet: AP-1 Reporter Activity Assay—Cells (107) in Medium B were transfected with 10 mg of irrelevant plasmid DNA (pcDNAIII; Invitrogen) and 20 mg of the AP-1 reporter plasmid, pGL-AP1-Luc (generously provided by E. O’Neill (Merck)), which contains three tandem repeats of a sequence (59-GTGACTCAGCGCG-39) with a high affinity AP-1 binding site (underlined) upstream of a minimal g-fibrinogen promoter (46) and a luciferase gene.

Plasmid Preparation:

Article Title: Gi Proteins Use a Novel βγ- and Ras-independent Pathway to Activate Extracellular Signal-regulated Kinase and Mobilize AP-1 Transcription Factors in Jurkat T Lymphocytes
Article Snippet: AP-1 Reporter Activity Assay—Cells (107) in Medium B were transfected with 10 mg of irrelevant plasmid DNA (pcDNAIII; Invitrogen) and 20 mg of the AP-1 reporter plasmid, pGL-AP1-Luc (generously provided by E. O’Neill (Merck)), which contains three tandem repeats of a sequence (59-GTGACTCAGCGCG-39) with a high affinity AP-1 binding site (underlined) upstream of a minimal g-fibrinogen promoter (46) and a luciferase gene.

Sequencing:

Article Title: Gi Proteins Use a Novel βγ- and Ras-independent Pathway to Activate Extracellular Signal-regulated Kinase and Mobilize AP-1 Transcription Factors in Jurkat T Lymphocytes
Article Snippet: AP-1 Reporter Activity Assay—Cells (107) in Medium B were transfected with 10 mg of irrelevant plasmid DNA (pcDNAIII; Invitrogen) and 20 mg of the AP-1 reporter plasmid, pGL-AP1-Luc (generously provided by E. O’Neill (Merck)), which contains three tandem repeats of a sequence (59-GTGACTCAGCGCG-39) with a high affinity AP-1 binding site (underlined) upstream of a minimal g-fibrinogen promoter (46) and a luciferase gene.

Binding Assay:

Article Title: Gi Proteins Use a Novel βγ- and Ras-independent Pathway to Activate Extracellular Signal-regulated Kinase and Mobilize AP-1 Transcription Factors in Jurkat T Lymphocytes
Article Snippet: AP-1 Reporter Activity Assay—Cells (107) in Medium B were transfected with 10 mg of irrelevant plasmid DNA (pcDNAIII; Invitrogen) and 20 mg of the AP-1 reporter plasmid, pGL-AP1-Luc (generously provided by E. O’Neill (Merck)), which contains three tandem repeats of a sequence (59-GTGACTCAGCGCG-39) with a high affinity AP-1 binding site (underlined) upstream of a minimal g-fibrinogen promoter (46) and a luciferase gene.

Luciferase:

Article Title: Gi Proteins Use a Novel βγ- and Ras-independent Pathway to Activate Extracellular Signal-regulated Kinase and Mobilize AP-1 Transcription Factors in Jurkat T Lymphocytes
Article Snippet: AP-1 Reporter Activity Assay—Cells (107) in Medium B were transfected with 10 mg of irrelevant plasmid DNA (pcDNAIII; Invitrogen) and 20 mg of the AP-1 reporter plasmid, pGL-AP1-Luc (generously provided by E. O’Neill (Merck)), which contains three tandem repeats of a sequence (59-GTGACTCAGCGCG-39) with a high affinity AP-1 binding site (underlined) upstream of a minimal g-fibrinogen promoter (46) and a luciferase gene.



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Merck & Co pgl-ap1-luc
Pgl Ap1 Luc, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl-ap1-luc/pgl+ap1+luc/10__1074_slash_jbc__274__28__19992-97-27-33
Average 90 stars, based on 1 article reviews
pgl-ap1-luc - by Bioz Stars, 2026-09
90/100 stars
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